Handbook of Microbiological Media, Fourth Edition part 38 is an invaluable reference for every medical, veterinary, diagnostic, and academic laboratory, and now in its fourth edition, it is even more complete. This edition carries on the tradition of CRC Press handbook excellence, listing the formulations, methods of preparation, and uses for more than 7000 microbiological media. With 1500 more entries than any previous edition, the handbook includes both classic and modern media used for the identification, cultivation, and maintenance of diverse bacteria, archaea, and fungi | Chopped Meat Broth 365 Preparation of Medium Add components to distilled deionized water and bring volume to . Mix thoroughly. Gently heat and bring to boiling. Continue boiling for 2-3 min. Allow precipitate to settle out. Distribute supernatant into flasks in volumes. Add standard solution or test solutions to each flask. Adjust the volume of each flask to with distilled deionized water. Autoclave for 10 min at 15 psi pressure-121 C. Use For the microbiological assay of choline using Neurospora crassa as the test microorganism. Choline Medium Composition per liter Choline pH at 25 C Preparation of Medium Add components to distilled deionized water and bring volume to . Mix thoroughly. Adjust pH to . Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure-121 C. Use For the cultivation of Achromobacter holinophagum. Chondromyces VYZ Agar Composition per liter Fresh baker s yeast pH at 25 C Preparation of Medium Add components to distilled deionized water and bring volume to . Mix thoroughly. Adjust pH to . Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure-121 C. Use For the cultivation of Chondromyces species. Chopped Liver Broth Composition per liter Fresh beef Soluble pH at 25 C Source This medium is available as a premixed powder from Hi-Media. Preparation of Medium Grind fresh beef liver. Add to of dis-tilled deionized water. Gently heat and bring to boiling. Continue boiling for 60 min. Cool to 25 C. Adjust pH to . Gently heat and bring to boiling. Continue boiling for 10 min. Filter through cheesecloth. Save chopped liver particles. To filtrate add remaining components. Bring volume to with distilled deionized water. Adjust pH to . Filter through Whatman 1 filter paper. Add chopped liver particles to
