Handbook of Microbiological Media, Fourth Edition part 39 is an invaluable reference for every medical, veterinary, diagnostic, and academic laboratory, and now in its fourth edition, it is even more complete. This edition carries on the tradition of CRC Press handbook excellence, listing the formulations, methods of preparation, and uses for more than 7000 microbiological media. With 1500 more entries than any previous edition, the handbook includes both classic and modern media used for the identification, cultivation, and maintenance of diverse bacteria, archaea, and fungi | Christensen s Urea Agar with Sodium Chloride 375 Preparation of Medium Add components to distilled deionized water and bring volume to . Mix thoroughly. Gently heat and bring to boiling. Dispense into tubes or flasks. Autoclave for 15 min at 15 psi pressure-121 C. Pour into sterile Petri dishes or leave in tubes. Allow tubes to cool in a slanted position. Use For the differentiation of enteric pathogens especially members of the Enterobacteriaceae and coliforms based on their ability to utilize citrate as a carbon source. Bacteria that can utilize citrate turn the medium pink-red. Christensen Citrate Sulfide Medium Composition per liter Sodium Yeast Ferric Ammonium Phenol pH at 25 C Preparation of Medium Add components to distilled deionized water and bring volume to . Mix thoroughly. Gently heat and bring to boiling. Dispense into tubes or flasks. Autoclave for 15 min at 15 psi pressure-121 C. Pour into sterile Petri dishes or leave in tubes. Allow tubes to cool in a slanted position. Use For the differentiation of enteric pathogens especially members of the Enterobacteriaceae and coliforms based on their ability to utilize citrate as a carbon source and production of H2S. Bacteria that can utilize citrate turn the medium pink-red. H2S production appears as a blackening of the butt of the tube. Christensen Citrate Sulfite Agar Composition per liter Sodium Yeast extract. Ferric ammonium citrate. Ammonium citrate . Glucose . Phenol Red. pH at 25 C Preparation of Medium Add components to distilled deionized water and bring volume to . Mix thoroughly. Gently heat and bring to boiling. Dispense into tubes or flasks. Autoclave for 15 min at 15 psi pressure-121 C. Pour
