Handbook of Microbiological Media, Fourth Edition part 40 is an invaluable reference for every medical, veterinary, diagnostic, and academic laboratory, and now in its fourth edition, it is even more complete. This edition carries on the tradition of CRC Press handbook excellence, listing the formulations, methods of preparation, and uses for more than 7000 microbiological media. With 1500 more entries than any previous edition, the handbook includes both classic and modern media used for the identification, cultivation, and maintenance of diverse bacteria, archaea, and fungi | Chromogenic Salmonella Esterase Agar 385 Preparation of Selective Supplement Solution Add components to distilled deionized water and bring volume to . Mix thoroughly. Filter sterilize. Preparation of Medium Add components except differential lecithin solution and selective supplement solution to distilled deionized water and bring volume to . Mix thoroughly. Gently heat while stirring and bring to boiling. Autoclave for 15 min at 15 psi pres-sure-121 C. Cool to 46 C. Aseptially add differential lecithin solution and selective supplement solution. Mix thoroughly. Pour into sterile Petri dishes. Use For the isolation enumeration and presumptive identification of Listeria spp. and Listeria monocytogenes. This selective medium contains the substrate lecithin which permits differentiation ofL. monocytogenes and L. Ivanovii from other Listeria species. Differential activity for all Listeria species is due to the addition of a chromogenic substrate. Chromogenic Listeria Agar ISO Composition per liter Enzymatic digest of animal Yeast Enzymatic digest of Na2HPO4 Sodium pyruvate . Magnesium MgSO4 X-glucoside chromogenic Selective supplement pH at 25 C Source This medium is available from Oxoid Unipath. Selective Supplement Solution Composition per Nalidixic Polymyxin 700 U Preparation of Selective Supplement Solution Add components to distilled deionized water and bring volume to . Mix thoroughly. Filter sterilize. Preparation of Medium Add components except L-a-phosphoti-dylinositol and selective supplement solution to distilled deionized water and bring volume to . Mix thoroughly. Gently heat while stirring and bring to boiling. Autoclave for 15 min at 15 psi pres-sure-121 C. Cool .
