Handbook of Microbiological Media, Fourth Edition part 43 is an invaluable reference for every medical, veterinary, diagnostic, and academic laboratory, and now in its fourth edition, it is even more complete. This edition carries on the tradition of CRC Press handbook excellence, listing the formulations, methods of preparation, and uses for more than 7000 microbiological media. With 1500 more entries than any previous edition, the handbook includes both classic and modern media used for the identification, cultivation, and maintenance of diverse bacteria, archaea, and fungi | Clostridium noterae Medium 415 Vitamin Solution Composition per liter Calcium Lipoic Nicotinic p-Aminobenzoic Folic Vitamin Preparation of Vitamin Solution Add components to distilled deionized water and bring volume to . Mix thoroughly. Preparation of Medium Add components except NaHCO3 solution and rhamnose solution to distilled deionized water and bring volume to . Mix thoroughly. Adjust pH to with KOH. Sparge under 100 N2 for 3-4 min. Autoclave for 15 min at 15 psi pressure-121 C. Aseptically and anaerobically add of sterile NaHCO3 solution and of sterile rhamnose solution. Mix thoroughly. Aseptically and anaerobically distribute into sterile screw-capped bottles under 100 N2. Final pH of the medium should be . Use For the cultivation of Clostridium methylpentosum. Clostridium neopropionicum Medium Composition per liter Pancreatic digest of Yeast g Trace elements Vitamin L-Cysteine-HCl-H2O Na2S-9H2O pH at 25 C Trace Elements Solution Composition per liter Nitrilotriacetic Preparation of Trace Elements Solution Add nitrilotriacetic acid to approximately of distilled deionized water. Dissolve by adding KOH and adjust pH to . Add remaining components. Bring volume to with additional distilled deionized water. Adjust pH to with KOH. Vitamin Solution Composition per liter Calcium Lipoic Nicotinic p-Aminobenzoic .
