Handbook of Microbiological Media, Fourth Edition part 48 is an invaluable reference for every medical, veterinary, diagnostic, and academic laboratory, and now in its fourth edition, it is even more complete. This edition carries on the tradition of CRC Press handbook excellence, listing the formulations, methods of preparation, and uses for more than 7000 microbiological media. With 1500 more entries than any previous edition, the handbook includes both classic and modern media used for the identification, cultivation, and maintenance of diverse bacteria, archaea, and fungi | CT Agar 465 Crystal 2 3 5-Triphenyltetrazolium chloride pH at 25 C Source This medium without triphenyltetrazolium chloride solution is available as a premixed powder from HiMedia. Caution Sodium azide is toxic. Azides also react with metals and disposal must be highly diluted. 2 3 5-Triphenyltetrazolium Chloride Solution Composition per 2 3 5-Triphenyltetrazolium Preparation of 2 3 5-Triphenyltetrazolium Chloride Solution Add 2 3 5-triphenyltetrazolium chloride to distilled deionized water and bring volume to . Mix thoroughly. Filter sterilize. Preparation of Medium Add components except 2 3 5-triphe-nyltetrazolium chloride solution to distilled deionized water and bring volume to . Mix thoroughly. Gently heat and bring to boiling. Autoclave for 10 min at 15 psi pressure-121 C. Cool to 45 -50 C. Aseptically add 2 3 5-triphenyltetrazolium chloride solution. Mix thoroughly. Pour into sterile Petri dishes or distribute into sterile tubes. Use For the detection of Gram-negative psychrotrophic bacteria causing food spoilage. CS Vitamin B12 Agar Composition per liter Sodium Vitamin assay casamino Papaic digest of soybean Sodium Ribonucleic Sorbitan monooleate Adenine Calcium Folic acid. Lactobacillus leichmannii pH at 25 C Preparation of Medium Add components except Lactobacillus leichmannii suspension to distilled deionized water and bring volume to . Mix thoroughly. Gently heat and bring to boiling. Autoclave for 15 min at 15 psi pressure-121 C. Cool to 45 -50
