Handbook of Microbiological Media, Fourth Edition part 54 is an invaluable reference for every medical, veterinary, diagnostic, and academic laboratory, and now in its fourth edition, it is even more complete. This edition carries on the tradition of CRC Press handbook excellence, listing the formulations, methods of preparation, and uses for more than 7000 microbiological media. With 1500 more entries than any previous edition, the handbook includes both classic and modern media used for the identification, cultivation, and maintenance of diverse bacteria, archaea, and fungi | Desulfohalobium Medium 525 NH4Cl . Yeast Resazurin . Wolfe s vitamin NaHCO3 Na2S-9H2O L-Cysteine-HCl Wolfe s Mineral n-Decane . pH at 25 C Solution Composition per Preparation of L-Cysteine Solution Add L-cysteine-HCl to dis-tilled deionized water and bring volume to . Mix thoroughly. Sparge with 100 N2. Autoclave under 100 N2 for 15 min at 15 psi pressure-121 C. Na2S-9H2O Solution Composition per Preparation of Na2S-9H2O Solution Add Na2S-9H2O to dis-tilled deionized water and bring volume to . Mix thoroughly. Autoclave under 100 N2 for 15 min at 15 psi pressure-121 C. Cool to room temperature. NaHCO3 Solution Composition per Preparation of NaHCO3 Solution Add components to distilled deionized water and bring volume to . Mix thoroughly. Sparge with 20 CO2 80 H2. Autoclave for 15 min at 15 psi pressure-121 C. Cool to room temperature. Wolfe s Mineral Elixir Composition per liter NH4 KAl SO4 Preparation of Wolfe s Mineral Elixir Adjust pH of dis-tilled deionized water to with dilute H2SO4. Add remaining components one at a time. Mix thoroughly to dissolve. Wolfe s Vitamin Solution Composition per liter Nicotinic Calcium p-Aminobenzoic Thioctic Folic Preparation of Wolfe s Vitamin Solution Add components to distilled deionized water and bring volume to . Mix thoroughly. Filter sterilize. Preparation of Medium Add components except .
