Handbook of Microbiological Media, Fourth Edition part 55 is an invaluable reference for every medical, veterinary, diagnostic, and academic laboratory, and now in its fourth edition, it is even more complete. This edition carries on the tradition of CRC Press handbook excellence, listing the formulations, methods of preparation, and uses for more than 7000 microbiological media. With 1500 more entries than any previous edition, the handbook includes both classic and modern media used for the identification, cultivation, and maintenance of diverse bacteria, archaea, and fungi | Desulfonema limicola Medium 535 Preparation of Solution F Vitamin Solution Add components to distilled deionized water and bring volume to . Mix thoroughly. Filter sterilize. Sparge with 100 N2. Solution G Artificial Sediment Composition per AlCl3-6H2O w v . Na2CO3 w v . Preparation of Solution G Artificial Sediment Combine components. Mix thoroughly. Sparge with 100 N2. Autoclave for 15 min at 15 psi pressure-121 C. Solution H Composition per Rumen fluid Preparation of Solution H Sparge with 100 N2. Autoclave for 15 min at 15 psi pressure-121 C. Solution I Composition per Preparation of Solution I Add Na2S-9H2O to distilled deionized water and bring volume to . Mix thoroughly. Sparge with 100 N2. Autoclave for 15 min at 15 psi pressure-121 C. Preparation of Medium Gently heat solution A and bring to boiling. Boil solution A for a few minutes. Cool to room temperature. Gas with 80 N2 20 CO2 gas mixture to reach a pH below 6. Autoclave for 15 min at 15 psi pressure-121 C. Add hot agar solution. Mix thoroughly. Cool to 50 C. Sequentially add solution B solution C solution D solution E solution F solution G solution H and solution I. Distribute anaerobically under 80 N2 20 CO2 into appropriate vessels. The pH should be . Addition of 10-20mg sodium dithionite per liter from a 5 w v solution freshly prepared under N2 and filter sterilized may stimulate growth. Use For the cultivation ofDesulfonema ishimotoi. Desulfonema limicola Medium DSMZ Medium 201 Composition per Solution Solution Solution Solution Solution F Vitamin solution . Solution Solution G Artificial sediment . Solution Solution B Trace elements solution SL-10 . pH at 25 C Solution A Resazurin .
