Handbook of Microbiological Media, Fourth Edition part 63 is an invaluable reference for every medical, veterinary, diagnostic, and academic laboratory, and now in its fourth edition, it is even more complete. This edition carries on the tradition of CRC Press handbook excellence, listing the formulations, methods of preparation, and uses for more than 7000 microbiological media. With 1500 more entries than any previous edition, the handbook includes both classic and modern media used for the identification, cultivation, and maintenance of diverse bacteria, archaea, and fungi | E Medium for Anaerobes 615 NH4 Yeast Preparation of Medium Add components to distilled deionized water and bring volume to . Mix thoroughly. Gently heat and bring to boiling. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure-121 C. Pour into sterile Petri dishes or leave in tubes. Use For the cultivation of Bipolaris sorghicola and Codinaea simplex. DYAA LUP Composition per liter Yeast FeCl3 Lupine FeCl3 Solution Composition per Preparation of FeClj Solution Add FeCl3 to distilled deionized water and bring volume to . Mix thoroughly. Preparation of Medium Add components except lupine stems to distilled deionized water and bring volume to . Mix thoroughly. Gently heat and bring to boiling. Distribute volumes into tubes. Cut lupine stems into pieces. Add 2-3 lupine stems per tube. Autoclave for 15 min at 15 psi pressure-121 C. Allow tubes to cool in a slanted position. Use For the cultivation of Ceratocystis coerulescens Ceratocystis microspora Coniella pulchella Phoma lini and Phoma glomerata. DYPA See Dextrose Yeast Extract Peptone E Agar m-E Agar Composition per liter Yeast Pancreatic digest of Nalidixic TTC pH at 25 C Caution Cycloheximide is toxic. Avoid skin contact or aerosol formation and inhalation. TTC Solution Composition per 2 3 5-Triphenyltetrazolium Preparation of TTC Solution Add triphenyltetrazolium chloride to distilled deionized water and bring volume to . Mix thoroughly. Filter sterilize. Preparation of Medium Add components except TTC solution to distilled deionized water and bring volume to . Mix thoroughly. Gently heat and
