Handbook of Microbiological Media, Fourth Edition part 65 is an invaluable reference for every medical, veterinary, diagnostic, and academic laboratory, and now in its fourth edition, it is even more complete. This edition carries on the tradition of CRC Press handbook excellence, listing the formulations, methods of preparation, and uses for more than 7000 microbiological media. With 1500 more entries than any previous edition, the handbook includes both classic and modern media used for the identification, cultivation, and maintenance of diverse bacteria, archaea, and fungi | Elliker Broth 635 Metals 44 Composition per ZnSO4-7H2O. Sodium Co NO3 Preparation of Metals 44 Add sodium EDTA to distilled de-ionized water and bring volume to . Mix thoroughly. Add a few drops of concentrated H2SO4 to retard precipitation of heavy metal ions. Add remaining components. Mix thoroughly. Bring volume to with distilled deionized water. Preparation of Hutner s Basal Salts Solution Add nitrilotriacetic acid to of distilled deionized water. Adjust pH to with KOH. Add remaining components. Add distilled deionized water to . Adjust pH to . Artificial Sea Water Composition per liter Na2SO4. . KBr. NaF. Preparation of Artificial Sea Water Add components to distilled deionized water and bring volume to . Mix thoroughly. Filter sterilize. Vitamin Solution Composition per liter p-Aminobenzoic Folic Vitamin Preparation of Vitamin Solution Add components to distilled deionized water and bring volume to . Mix thoroughly. Filter sterilize. Store in the dark at 5 C. Glucose Solution Composition per Preparation of Glucose Solution Add glucose to distilled deionized water and bring volume to . Mix thoroughly. Filter sterilize. Preparation of Medium Add components except artificial sea water glucose solution and vitamin solution to distilled deionized water and bring volume to . Mix thoroughly. Adjust pH to . Autoclave for 20 min at 15 psi pressure-121 C. Cool to 60 C. Warm artificial sea water to 55 C. Aseptically add warm artificial sea water. Mix thoroughly. Adjust pH to . Aseptically add glucose .
