Handbook of Microbiological Media, Fourth Edition part 69 is an invaluable reference for every medical, veterinary, diagnostic, and academic laboratory, and now in its fourth edition, it is even more complete. This edition carries on the tradition of CRC Press handbook excellence, listing the formulations, methods of preparation, and uses for more than 7000 microbiological media. With 1500 more entries than any previous edition, the handbook includes both classic and modern media used for the identification, cultivation, and maintenance of diverse bacteria, archaea, and fungi | Ferrous Sulfate Yeast Extract Medium 675 Vitamin Solution Composition per liter Nicotinic p-Aminobenzoic Lipoic Folic Vitamin Preparation of Vitamin Solution Add components to distilled deionized water and bring volume to . Mix thoroughly. Sparge with 80 H2 20 CO2. Filter sterilize. Na2S-9H2O Solution Composition per Preparation of Na2S-9H2O Solution Add Na2S-9H2O to dis-tilled deionized water and bring volume to . Sparge with N2. Autoclave for 15 min at 15 psi pressure-121 C. Cool to 25 C. Store anaerobically. Preparation of Medium Add components except Na2S-9H2O solution vitamin solution and Na-pyruvate solution to distilled deion-ized water and bring volume to . Mix thoroughly. Flush medium with N2 for 20 min. Adjust medium pH to with H2SO4. Add of Na2S-9H2O solution. Mix thoroughly. Readjust the medium pH to with H2SO4 while flushing the gas phase only with N2. Dispense 10mL volumes into 100mL serum bottles with rubber stoppers under N2. Replace gas phase by 80 H2 20 CO2 gas mixture and finally pressurize the bottles to 2 bar gas overpressure. Autoclave for 15 min at 15 psi pressure-121 C. Aseptically inject via syringe vitamin solution and Na-pyruvate solution into each tube containing of the autoclaved medium. Use For the cultivation of Ferroglobus placidus. Ferroplasma acidiphilum Medium DSMZ Medium 874 Composition per Solution Solution Solution pH at 25 C Solution A Composition per NH4 Preparation of Solution A Add components to distilled deionized water and bring volume to . Mix thoroughly. Solution B Composition per H2SO4 Preparation of Solution B Add components to distilled deionized water
