Handbook of Microbiological Media, Fourth Edition part 70 is an invaluable reference for every medical, veterinary, diagnostic, and academic laboratory, and now in its fourth edition, it is even more complete. This edition carries on the tradition of CRC Press handbook excellence, listing the formulations, methods of preparation, and uses for more than 7000 microbiological media. With 1500 more entries than any previous edition, the handbook includes both classic and modern media used for the identification, cultivation, and maintenance of diverse bacteria, archaea, and fungi | Flexibacter Broth 685 Sodium Preparation of Trace Elements Solution HO-LE Add components to distilled deionized water and bring volume to . Mix thoroughly. Filter sterilize. Preparation of Medium Add components except traqce elements solution HO-LE to filtered seawater and bring volume to . Mix thoroughly. Gently heat and bring to boiling. Autoclave for 15 min at 15 psi pressure-121 C. Cool to 45 -50 C. Aseptically add of sterile trace elements solution HO-LE. Mix thoroughly. Pour into sterile Petri dishes or distribute into sterile tubes. Use For the cultivation and maintenance of Flexibacter polymorphus. Flexibacter Agar LMG Medium 60 Composition per liter Casamino Tris Vitamin Glucose Vitamin Trace elements pH at 25 C Glucose Solution Composition per Preparation of Glucose Solution Add glucose to distilled deionized water and bring volume to . Mix thoroughly. Filter sterilize. Trace Elements Solution Composition per liter Sodium Preparation of Trace Elements Solution Add components to distilled deionized water and bring volume to . Mix thoroughly. Filter sterilize. Vitamin Solution Composition per Vitamin Preparation of Vitamin Solution Add components to distilled deionized water and bring volume to . Mix thoroughly. Filter sterilize. Preparation of Medium Add components except vitamin solution glucose solution and trace elements solution to distilled deionized water. Mix thoroughly. Gently heat and bring to boiling. Autoclave for 15 min at 15 psi pressure-121 C.
