Handbook of Microbiological Media, Fourth Edition part 73 is an invaluable reference for every medical, veterinary, diagnostic, and academic laboratory, and now in its fourth edition, it is even more complete. This edition carries on the tradition of CRC Press handbook excellence, listing the formulations, methods of preparation, and uses for more than 7000 microbiological media. With 1500 more entries than any previous edition, the handbook includes both classic and modern media used for the identification, cultivation, and maintenance of diverse bacteria, archaea, and fungi | FWM Medium 715 tion to distilled deionized water and bring volume to . Mix thoroughly. Gently heat and bring to boiling. Autoclave for 15 min at 15 psi pressure-121 C. Cool to 45 -50 C. Aseptically add of sterile egg yolk emulsion 50 of Crystal Violet solution and of sterile phenylethyl alcohol solution. Mix thoroughly. Pour into sterile Petri dishes or distribute into sterile tubes. Use For the isolation and cultivation ofFusobacterium necrophorum. FWM Medium Composition per Solution Solution E NaHCO3 solution . Solution F Substrate solution . Solution G Na2S-9H2O solution . Solution B Trace elements solution SL-10 . Solution C Seven vitamin solution . Solution D Selenite-tungstate solution . pH at 25 C Solution A Composition per Preparation of Solution A Prepare and dispense under 80 N2 20 CO2. Add components to distilled deionized water and bring volume to . Mix thoroughly. Autoclave for 15 min at 15 psi pres-sure-121 C. Solution B Trace Elements Solution SL-10 Composition per liter HCl 25 solution . Preparation of Solution B Trace Elements Solution SL-10 Prepare and dispense under 100 N2. Add FeCl2-4H2O to of HCl solution. Mix thoroughly. Add distilled deionized water and bring volume to . Add remaining components. Mix thoroughly. Autoclave for 15 min at 15 psi pressure-121 C. Solution C Seven Vitamin Solution Composition per liter Nicotinic Calcium p-Aminobenzoic d Preparation of Solution C Seven Vitamin Solution Add components to distilled deionized water and bring volume to . Mix thoroughly.
