Handbook of Microbiological Media, Fourth Edition part 75 is an invaluable reference for every medical, veterinary, diagnostic, and academic laboratory, and now in its fourth edition, it is even more complete. This edition carries on the tradition of CRC Press handbook excellence, listing the formulations, methods of preparation, and uses for more than 7000 microbiological media. With 1500 more entries than any previous edition, the handbook includes both classic and modern media used for the identification, cultivation, and maintenance of diverse bacteria, archaea, and fungi | Geobacter Medium 735 Preparation of Medium Add components to distilled deionized water and bring volume to . Mix thoroughly. Gently heat and bring to boiling. Adjust pH to . Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure-121 C. Pour into sterile Petri dishes or leave in tubes. Use For the cultivation of Geodermatophilus obscurus. Geoalkalibacter Medium DSMZ Medium 1059 Composition per liter Sulfur NH4Cl . Yeast Sodium acetate Trace elements solution Selenite tungstate pH at 25 C Sodium Acetate Solution Composition per Sodium Preparation of Sodium Acetate Solution Add components to distilled deionized water and bring volume to . Mix thoroughly. Sparge with 100 N2. Autoclave for 15 min at 15 psi pressure-121 C. Trace Elements Solution SL-10 Composition per liter HCl 25 solution . Preparation of Trace Elements Solution SL-10 Add FeCl2-4H2O to of HCl solution. Mix thoroughly. Add distilled deionized water and bring volume to . Add remaining components. Sparge with 100 N2. Autoclave for 15 min at 15 psi pressure-121 C. Selenite Tungstate Solution Composition per liter Preparation of Selenite Tungstate Solution Add components to distilled deionized water and bring volume to . Mix thoroughly. Sparge with 100 N2. Autoclave for 15 min at 15 psi pressure-121 C. Preparation of Medium Add components except sulfur carbonate sodium bicarbonate and sodium acetate solution to distilled de-ionized water and bring volume to . Gently heat and bring to boiling. Boil for 1 min. Cool to room temperature under N2 gas atmo sphere. Add solid carbonate and .
