Handbook of Microbiological Media, Fourth Edition part 76 is an invaluable reference for every medical, veterinary, diagnostic, and academic laboratory, and now in its fourth edition, it is even more complete. This edition carries on the tradition of CRC Press handbook excellence, listing the formulations, methods of preparation, and uses for more than 7000 microbiological media. With 1500 more entries than any previous edition, the handbook includes both classic and modern media used for the identification, cultivation, and maintenance of diverse bacteria, archaea, and fungi | Glucose Nitrogen-Free Salt Agar 745 Glucose Cysteine HiVeg Agar Base with Thiamine and Blood Composition per liter Papaic digest of soybean Plant peptone No. Blood pH at 25 C Source This medium without blood is available as a premixed powder from HiMedia. Preparation of Medium Add components except blood to dis-tilled deionized water and bring volume to . Mix thoroughly. Gently heat until boiling. Autoclave for 15 min at 15 psi pressure-121 C. Cool to 75 -80 C. Add sterile defibrinated blood with thorough mixing and maintain at 75 -80 C for 15-20 min until the medium is chocolatized. Pour into sterile Petri dishes or distribute into sterile tubes. Use For the cultivation and enumeration of Pasteurella tularensis. Glucose HiVeg Broth Composition per liter Plant Source This medium is available from HiMedia. Preparation of Medium Add components to distilled deionized water and bring volume . Mix thoroughly. Gently heat and bring to boiling. Cool to 25 . Adjust pH to . Distribute into sterile tubes or flasks. Autoclave for 15 min at 12 psi pressure-118 C. Use For the study of glucose fermentation where pH indicator is not desired. Glucose HiVeg Peptone Agar Composition per liter Plant pH at 25 C Source This medium is available as a premixed powder from Hi-Media. Preparation of Medium Add components to distilled deionized water and bring volume to . Mix thoroughly. Gently heat and bring to boiling. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure-121 C. Pour into sterile Petri dishes or leave in tubes. Use For the cultivation of Agrobacterium species. Glucose Medium Nakayama DSMZ Medium 452 Composition per liter Yeast Solution Solution .
