Handbook of Microbiological Media, Fourth Edition part 78 is an invaluable reference for every medical, veterinary, diagnostic, and academic laboratory, and now in its fourth edition, it is even more complete. This edition carries on the tradition of CRC Press handbook excellence, listing the formulations, methods of preparation, and uses for more than 7000 microbiological media. With 1500 more entries than any previous edition, the handbook includes both classic and modern media used for the identification, cultivation, and maintenance of diverse bacteria, archaea, and fungi | GV Medium 765 Trace Elements Solution SL-10 Composition per liter HCl 25 solution . Preparation of Trace Elements Solution SL-10 Add FeCl2-4H2O to of HCl solution. Mix thoroughly. Add distilled deionized water and bring volume to . Add remaining components. Mix thoroughly. Gas under 80 N2 20 CO2. Autoclave for 15 min at 15 psi pressure-121 C. Preparation of Medium Prepare and dispense medium under 80 N2 20 CO2. Add components except substrate solution MgSO4-7H2O solution CaCl2-2H2O solution selenite-tungstate solution L-cysteine-HCl solution and Na2S-9H2O solution to distilled deionized water and bring volume to . Mix thoroughly. Sparge with 80 N2 20 CO2. Autoclave for 15 min at 15 psi pressure-121 C. Aseptically and anaerobically add of sterile substrate solution of sterile MgSO4-7H2O solution of sterile CaCl2-2H2O solution of sterile selenite-tungstate solution of sterile L-cysteine-HCl solution and of sterile Na2S-9H2O solution. Mix thoroughly. Adjust pH to . Aseptically and anaerobically distribute into sterile tubes or flasks. Use For the cultivation and maintenance of Clostridium magnum and Clostridium species. GV Medium Composition per Yeast Substrate Vitamin L-Cysteine-HCl Na2S-9H2O MgSO4-7H2O CaCl2-2H2O Selenite-tungstate Trace elements solution pH at 25 C Substrate Solution Composition per Sodium Preparation of Substrate Solution Add sodium citrate to distilled deionized water and bring volume to . Mix thoroughly. Sparge with 80 N2 20 CO2. Autoclave for 15 min at 15 psi pres-sure-121 C. .
