Handbook of Microbiological Media, Fourth Edition part 82 is an invaluable reference for every medical, veterinary, diagnostic, and academic laboratory, and now in its fourth edition, it is even more complete. This edition carries on the tradition of CRC Press handbook excellence, listing the formulations, methods of preparation, and uses for more than 7000 microbiological media. With 1500 more entries than any previous edition, the handbook includes both classic and modern media used for the identification, cultivation, and maintenance of diverse bacteria, archaea, and fungi | Halothermothrix orenii Medium 805 Preparation of NaHCO3 Solution Add components to distilled deionized water and bring volume to . Mix thoroughly. Sparge with 20 CO2 80 H2. Autoclave for 15 min at 15 psi pressure-121 C. Cool to room temperature. Vitamin Solution V7 Composition per liter Nicotinic Vitamin p-Aminobenzoic Preparation of Vitamin Solution V7 Add components to dis-tilled deionized water and bring volume to . Mix thoroughly. Filter sterilize. Trace Elements Solution SL-12 Composition per liter ZnCl2. Preparation of Trace Elements Solution SL-12 Add components. to distilled deionized water and bring volume to . Mix thoroughly. Filter sterilize. Preparation of Medium Add components except sulfide bicarbonate vitamin and trace elements solutions to distilled deionized water and bring volume to . Mix thoroughly. Adjust pH to . Gently heat while stirring and bring to boiling. Autoclave for 20 min at 15 psi pressure-121 C. Cool to room temperature. Aseptically add sulfide bicarbonate vitamin and trace elements solutions. Mix thoroughly. Aseptically distribute into sterile screw-cap tubes bottles or flasks. Use For the improved growth during cultivation of pure cultures of Halorhodospira spp. Halorubrum californiense Medium DSMZ Medium 1194 Composition per liter Yesat pH at 25 C Preparation of Medium Add components to distilled deionized water and bring volume to . Mix thoroughly. Adjust pH to . Gently heat while stirring and bring to boiling. Mix thoroughly. Distribute into tubes bottles or flasks. Autoclave for 15 min at 15 psi pressure-121 C. Use For the cultivation of Halorubrum .
