Handbook of Microbiological Media, Fourth Edition part 83 is an invaluable reference for every medical, veterinary, diagnostic, and academic laboratory, and now in its fourth edition, it is even more complete. This edition carries on the tradition of CRC Press handbook excellence, listing the formulations, methods of preparation, and uses for more than 7000 microbiological media. With 1500 more entries than any previous edition, the handbook includes both classic and modern media used for the identification, cultivation, and maintenance of diverse bacteria, archaea, and fungi | Heart Infusion Broth with Human Serum and Fresh Yeast Extract 815 Preparation of Glucose Solution Add D-glucose to distilled de-ionized water and bring volume to . Mix thoroughly. Filter sterilize. Preparation of Medium Add components except antibiotic inhibitor solution and glucose solution to distilled deionized water and bring volume to . Mix thoroughly. Gently heat and bring to boiling. Autoclave for 15 min at 15 psi pressure-121 C. Cool to 45 -50 C. Aseptically add sterile antibiotic inhibitor solution and glucose solution. Mix thoroughly. Aseptically distribute into sterile tubes or flasks. Use For the cultivation of Escherichia coli. Heart Infusion Broth HiVeg Composition per liter Plant hydrolysate No. Plant pH at 25 C Source This medium is available as a premixed powder from Hi-Media. Preparation of Medium Add components to distilled deionized water and bring volume to . Mix thoroughly. Gently heat and bring to boiling. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure-121 C. Use For the isolation and cultivation of a wide variety of fastidious microorganisms. Heart Infusion Broth with Horse Serum and Fresh Yeast Extract Composition per Heart infusion Horse serum Fresh yeast extract pH at 25 C Heart Infusion Broth Composition per liter Beef heart infusion Preparation of Heart Infusion Broth Add components to dis-tilled deionized water and bring volume to . Mix thoroughly. Autoclave for 15 min at 15 psi pressure-121 C. Cool to 45 -50 C. Fresh Yeast Extract Solution Composition per Baker s yeast live pressed Preparation of Fresh Yeast Extract Solution Add the live Baker s yeast to of distilled deionized water. Autoclave for 90 min at 15 psi pressure-121 C. Allow to stand. Remove supernatant solution. Adjust pH to . Preparation of Medium
