Handbook of Microbiological Media, Fourth Edition part 84 is an invaluable reference for every medical, veterinary, diagnostic, and academic laboratory, and now in its fourth edition, it is even more complete. This edition carries on the tradition of CRC Press handbook excellence, listing the formulations, methods of preparation, and uses for more than 7000 microbiological media. With 1500 more entries than any previous edition, the handbook includes both classic and modern media used for the identification, cultivation, and maintenance of diverse bacteria, archaea, and fungi | HESP1 SR1 TMC4 LUP Medium 825 NaHCO3 Substrate Na2S-9H2O Trace elements solution pH at 25 C Trace Elements Solution SL-10 Composition per liter HCl 25 solution . Preparation of Trace Elements Solution SL-10 Add FeCl24H2O to of HCl solution. Mix thoroughly. Add distilled deionized water and bring volume to . Add remaining components. Mix thoroughly. Sparge with 100 N2. Autoclave for 15 min at 15 psi pressure-121 C. NaHCO3 Solution Composition per Preparation of NaHCO3 Solution Add NaHCO3 to distilled deionized water and bring volume to . Mix thoroughly. Sparge with 80 N2 20 CO2. Filter sterilize. Na2S-9H2O Solution Composition per Preparation of Na2S-9H2O Solution Add Na2S-9H2O to dis-tilled deionized water and bring volume to . Sparge with N2. Autoclave for 15 min at 15 psi pressure-121 C. Cool to 25 C. Store anaerobically. Substrate Solution Composition per Preparation of Substrate Solution Add Na2S2O3-5H2O to dis-tilled deionized water and bring volume to . Sparge with N2. Filter sterilize. Preparation of Medium Prepare and dispense medium under 80 N2 20 CO2. Add components except NaHCO3 solution Na2S-9H2O solution and substrate solution to distilled deionized water and bring volume to . Mix thoroughly. Adjust pH to . Gently heat and bring to boiling. Boil for 10 min. Cool to room temperature while sparging with 80 N2 20 CO2. Adjust pH to . Dispense under 80 N2 20 CO2 into tubes or bottles. Autoclave for 15 min at 15 psi pressure-121 C. Cool to 25 C. Aseptically and anaerobically add sterile NaHCO3 solution per medium Na2S-9H2O solution per medium and substrate solution per medium. Final pH is
