Handbook of Microbiological Media, Fourth Edition part 88 is an invaluable reference for every medical, veterinary, diagnostic, and academic laboratory, and now in its fourth edition, it is even more complete. This edition carries on the tradition of CRC Press handbook excellence, listing the formulations, methods of preparation, and uses for more than 7000 microbiological media. With 1500 more entries than any previous edition, the handbook includes both classic and modern media used for the identification, cultivation, and maintenance of diverse bacteria, archaea, and fungi | Hyperthermus butylicus Medium 865 Sparge with 80 N2 20 CO2. Autoclave for 15 min at 15 psi pres-sure-121 C. Solution F Composition per Sodium Preparation of Solution F Add sodium dihydroxybenzoate to dis-tilled deionized water and bring volume to . Mix thoroughly. Filter sterilize. Sparge with 80 N2 20 CO2. Solution G Composition per Preparation of Solution G Add Na2S-9H2O to distilled deionized water and bring volume to . Mix thoroughly. Sparge with 80 N2 20 CO2. Autoclave for 15 min at 15 psi pressure-121 C. Preparation of Medium Prepare and dispense medium under 80 N2 20 CO2. Aseptically and anaerobically combine of sterile solution A of sterile solution B of sterile solution C of sterile solution D of sterile solution E of sterile solution F and of sterile solution G. Mix thoroughly. Aseptically and anaerobically distribute into sterile tubes or flasks. Use For the cultivation and maintenance of Clostridium species. Hydroxybenzoic Acid Medium Composition per liter 4-Hydroxybenzoic Nitrilotriacetic pH at 25 C Preparation of Medium Add 4-hydroxybenzoic acid and nitrilotriacetic acid to approximately of distilled deionized water. Adjust pH to with concentrated NaOH. Add remaining components. Mix thoroughly. Readjust pH to . Bring volume to with distilled deionized water. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure-121 C. Use For the cultivation and maintenance of Bacillus species. Hydroxybutyrate Medium 3HB Medium LMG Medium 186 Composition per liter Ferric ammonium Yeast Buffer pH at 25 C Buffer Solution .
