Handbook of Microbiological Media, Fourth Edition part 96 is an invaluable reference for every medical, veterinary, diagnostic, and academic laboratory, and now in its fourth edition, it is even more complete. This edition carries on the tradition of CRC Press handbook excellence, listing the formulations, methods of preparation, and uses for more than 7000 microbiological media. With 1500 more entries than any previous edition, the handbook includes both classic and modern media used for the identification, cultivation, and maintenance of diverse bacteria, archaea, and fungi | Leptospira Protein-Free Medium 945 Leptospira enrichment EMJH a solution of albumin polysorbate 80 and additional growth factors available from BD pH at 25 C Preparation of Medium Add agarose to distilled deionized water and bring volume to . Mix thoroughly. Gently heat while stirring and bring to boiling. Boil with mixing until agarose dissolves. Add the other components except Leptospira enrichment EMJH. Mix thoroughly. Autoclave for 15 min at 15 psi pressure-121 C. Cool to 50 C. Mix thoroughly. Warm the Leptospira enrichment to approximately 25 C and add to the warm medium. Mix thoroughly. Aseptically distribute into sterile tubes or flasks. Use For the cultivation of Leptospira spp. Leptospira Medium EMJH Leptospira Medium Ellinghausen-McCullough Johnson-Harris Composition per liter Rabbit pH at 25 C Source This medium is available as a premixed powder from BD Diagnostic Systems. Preparation of Medium Add components except rabbit serum to distilled deionized water and bring volume to . Mix thoroughly. Gently heat and bring to boiling. Autoclave for 15 min at 15 psi pressure-121 C. Cool to 25 C. Aseptically add sterile rabbit serum. Mix thoroughly. Aseptically distribute into sterile tubes or flasks. Use For the cultivation and maintenance of Leptospira species. Leptospira Medium Modified Composition per liter Beef extract . Hemin Sterile rabbit pH at 25 C Hemin Solution Composition per NaOH 1jV solution . Preparation of Hemin Solution Add hemin to of 1jV NaOH solution. Mix thoroughly. Bring volume to with distilled deionized water. Autoclave for 15 min at 15 psi pressure-121 C. Cool to 45 -50 C. Preparation of Medium Add components except hemin solution and rabbit serum to .
