Handbook of Microbiological Media, Fourth Edition part 106 is an invaluable reference for every medical, veterinary, diagnostic, and academic laboratory, and now in its fourth edition, it is even more complete. This edition carries on the tradition of CRC Press handbook excellence, listing the formulations, methods of preparation, and uses for more than 7000 microbiological media. With 1500 more entries than any previous edition, the handbook includes both classic and modern media used for the identification, cultivation, and maintenance of diverse bacteria, archaea, and fungi | Medium for Chlorobium ferrooxidans 1045 HCl 25 solution . Preparation of Trace Elements Solution SL-10 Add FeCl2-4H2O to of HCl solution. Mix thoroughly. Add distilled deionized water and bring volume to . Add remaining components. Mix thoroughly. Sparge with 100 N2. Autoclave for 15 min at 15 psi pressure-121 C. Cool to room temperature. Preparation of Medium Aseptically and anaerobically combine solution A solution B and solution C. Aseptically and anaerobically add vitamin solution and trace elements solution SL-10. Mix thoroughly. The pH should be . Use For the cultivation of Dechloromonas agitata and Azospira oryzae. Medium with Chloroacrylic Acid DSMZ Medium 457c Composition per liter NH4 Chloroacrylic acid Trace elements solution pH at 25 C Trace Elements Solution SL-4 Composition per liter Trace elements solution Trace Elements Solution SL-6 Composition per liter Preparation of Trace Elements Solution SL-6 Add components to distilled deionized water and bring volume to . Mix thoroughly. Adjust pH to . Preparation of Trace Elements Solution SL-4 Add components to distilled deionized water and bring volume to . Mix thoroughly. Chloroacrylic Acid Solution Composition per liter 3-Chloroacrylic Preparation of Chloroacrylic Acid Solution Add 3-chloro-acrylic acid to distilled deionized water and bring volume to . Mix thoroughly. Adjust pH to . Filter sterilize. Preparation of Medium Add components except chloroacrylic acid solution to distilled deionized water. Adjust pH to . Autoclave for 15 min at 15 psi pressure-121 C. Cool to room temperature. Aseptically
