Handbook of Microbiological Media, Fourth Edition part 112 is an invaluable reference for every medical, veterinary, diagnostic, and academic laboratory, and now in its fourth edition, it is even more complete. This edition carries on the tradition of CRC Press handbook excellence, listing the formulations, methods of preparation, and uses for more than 7000 microbiological media. With 1500 more entries than any previous edition, the handbook includes both classic and modern media used for the identification, cultivation, and maintenance of diverse bacteria, archaea, and fungi | Methanogenium thermophilicum Medium 1105 Vitamin L-Cysteine-HCl-H2O Na2S-9H2O pH at 25 C Trace Elements Solution Composition per liter Nitrilotriacetic KAl SO4 Preparation of Trace Elements Solution Add nitrilotriacetic acid to of distilled deionized water. Adjust pH to with KOH. Add remaining components. Add distilled deionized water to . Adjust pH to with KOH. Vitamin Solution Composition per liter Nicotinic p-Aminobenzoic Lipoic Folic Vitamin Preparation of Vitamin Solution Add components to distilled deionized water and bring volume to . Mix thoroughly. Filter sterilize. L-Cysteine-HCbH2O Solution Composition per Preparation of L-Cysteine-HCbH2O Solution Add L-cyste-ine-HCl-H2O to distilled deionized water and bring volume to . Mix thoroughly. Sparge with 100 N2. Autoclave for 15 min at 15 psi pressure-121 C. Cool to room temperature. Na2S-9H2O Solution Composition per Preparation of Na2S-9H2O Solution Add Na2S-9H2O to dis-tilled deionized water and bring volume to . Mix thoroughly. Autoclave under 100 N2 for 15 min at 15 psi pressure-121 C. Cool to room temperature. Preparation of Medium Prepare and dispense medium under 80 H2 20 CO2 gas atmosphere. Add components except L-cysteine-HCl-H2O solution Na2S-9H2O solution and vitamin solution to distilled deionized water and bring volume to . Mix thoroughly. Adjust pH to . Sparge with 80 H2 20 CO2. Autoclave for 15 min at 15 psi pressure-121 C. Aseptically and anaerobically add .
