Handbook of Microbiological Media, Fourth Edition part 117 is an invaluable reference for every medical, veterinary, diagnostic, and academic laboratory, and now in its fourth edition, it is even more complete. This edition carries on the tradition of CRC Press handbook excellence, listing the formulations, methods of preparation, and uses for more than 7000 microbiological media. With 1500 more entries than any previous edition, the handbook includes both classic and modern media used for the identification, cultivation, and maintenance of diverse bacteria, archaea, and fungi | Micro Assay Culture Agar 1155 NaHCO3 pH at 25 C NaHCO3 Solution Composition per Preparation of NaHCO3 Solution Add NaHCO3 to distilled de-ionized water and bring volume to . Mix thoroughly. Filter sterilize. Preparation of Medium Add components except NaHCO3 solution to distilled deionized water and bring volume to . Mix thoroughly. Autoclave for 15 min at 15 psi pressure-121 C. Aseptically add of sterile NaHCO3 solution. Mix thoroughly. Aseptically distribute into sterile tubes or flasks. Use For the cultivation of Zygomonas mobilis Salinicoccus hispani-cus Salinicoccus roseus and Pseudomonas beijerinckii. MH Medium 15 LMG Medium 258 Composition per liter Yeast Proteose peptone NaHCO3 pH at 25 C NaHCO3 Solution Composition per Preparation of NaHCO3 Solution Add NaHCO3 to of distilled deionized water. Mix thoroughly. Filter sterilize. Preparation of Medium Add components except NaHCO3 solution to distilled deionized water and bring volume to . Mix thoroughly. Autoclave for 15 min at 15 psi pressure-121 C. Aseptically add sterile NaHCO3 solution. Aseptically distribute into sterile tubes or flasks. Use For cultivation and maintenance of Bacillus halophilus. MH Salts Composition per liter Agar . Yeast Proteose peptone No. pH at 25 C Preparation of Medium Add components to distilled deionized water and bring volume to . Mix thoroughly. Adjust pH to . Gently heat and bring to boiling. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure-121 C. Pour into sterile Petri dishes or leave in tubes. Use For the cultivation and maintenance of Bacillus .
