Handbook of Microbiological Media, Fourth Edition part 121 is an invaluable reference for every medical, veterinary, diagnostic, and academic laboratory, and now in its fourth edition, it is even more complete. This edition carries on the tradition of CRC Press handbook excellence, listing the formulations, methods of preparation, and uses for more than 7000 microbiological media. With 1500 more entries than any previous edition, the handbook includes both classic and modern media used for the identification, cultivation, and maintenance of diverse bacteria, archaea, and fungi | MJANHOX-NO3 Medium with Supplement 1195 Preparation of Bicarbonate Solution Add NaHCO3 to distilled deionized water and bring volume to . Mix thoroughly. Filter sterilize. Thiosulfate Solution Composition per Preparation of Thiosulfate Solution Add NaS2O3-5H2O to dis-tilled deionized water and bring volume to . Mix thoroughly. Autoclave for 15 min at 15 psi pressure-121 C. Cool to room temperature. Trace Elements Solution Composition per liter Nitrilotriacetic KAl SO4 Preparation of Trace Elements Solution Add nitrilotriacetic acid to of distilled deionized water. Dissolve by adjusting pH to with KOH. Add remaining components. Add distilled deionized water to . Mix thoroughly. Vitamin Solution Composition per liter Nicotinic p-Aminobenzoic Lipoic Folic Vitamin Preparation of Vitamin Solution Add components to distilled deionized water and bring volume to . Mix thoroughly. Sparge with 80 H2 20 CO2. Filter sterilize. Preparation of Medium Add components except thiosulfate bicarbonate and vitamin solutions to distilled deionized water and bring volume to . Mix thoroughly. Gently heat and bring to boiling. Boil for 1 min. Cool to room temperature while sparging with a gas mixture of 80 N2 20 CO2. Dispense under the same atmosphere into culture vessels. Fill up to a volume of 20 volume of vessel. Autoclave for 15 min at 15 psi pressure-121 C. Cool to room temperature under an atmosphere of 80 N2 20 CO2. Aseptically add sterile thiosulfate bicarbonate and vitamin solutions. Mix thoroughly. Adjust pH to . Aseptically dispense into tubes flasks or
