Handbook of Microbiological Media, Fourth Edition part 122 is an invaluable reference for every medical, veterinary, diagnostic, and academic laboratory, and now in its fourth edition, it is even more complete. This edition carries on the tradition of CRC Press handbook excellence, listing the formulations, methods of preparation, and uses for more than 7000 microbiological media. With 1500 more entries than any previous edition, the handbook includes both classic and modern media used for the identification, cultivation, and maintenance of diverse bacteria, archaea, and fungi | Modified FWM Medium 1205 Solution Solution Solution Solution pH at 25 C Solution A Composition per Preparation of Solution A Prepare under 80 N2 20 CO2 gas atmosphere. Add components to distilled deionized water and bring volume to . Mix thoroughly. Adjust pH to . Sparge with 80 N2 20 CO2. Autoclave for 15 min at 15 psi pressure-121 C. Cool to 25 C. Solution B Composition per liter HCl 25 solution . Preparation of Solution B Add FeCl2-4H2O to of HCl solution. Mix thoroughly. Add distilled deionized water and bring volume to . Add remaining components. Mix thoroughly. Sparge with 80 N2 20 CO2. Autoclave for 15 min at 15 psi pressure-121 C. Cool to 25 C. Solution C Composition per liter Pyridoxine Nicotinic Vitamin Calcium p-Aminobenzoic D Preparation of Solution C Add components to distilled deionized water and bring volume to . Sparge with 100 N2. Mix thoroughly. Filter sterilize. Solution D Composition per liter Preparation of Solution D Add components to distilled deionized water and bring volume to . Mix thoroughly. Sparge with 100 N2. Filter sterilize. Solution E Composition per Preparation of Solution E Add NaHCO3 to distilled deionized water and bring volume to . Mix thoroughly. Sparge with 100 N2 gas mixture. Autoclave for 15 min at 15 psi pressure-121 C. Cool to 25 C. Solution F Composition per Fructose. Preparation of Solution F Add fructose to distilled deionized water and bring volume to . Mix thoroughly. Sparge with 100 N2 gas mixture. Autoclave
