Handbook of Microbiological Media, Fourth Edition part 140 is an invaluable reference for every medical, veterinary, diagnostic, and academic laboratory, and now in its fourth edition, it is even more complete. This edition carries on the tradition of CRC Press handbook excellence, listing the formulations, methods of preparation, and uses for more than 7000 microbiological media. With 1500 more entries than any previous edition, the handbook includes both classic and modern media used for the identification, cultivation, and maintenance of diverse bacteria, archaea, and fungi | Phenol Red Broth Base with Plant Extract No. 1 1385 Beef Phenol pH at 25 C Source This medium is available from HiMedia. Preparation of Medium Add components to distilled deionized water and bring volume to . Mix thoroughly. Distribute into tubes with inverted Durham tubes. Autoclave for 15 min at 15 psi pressure-121 C. Use For the detection of adonitol fermenting bacteria. Phenol Red Agar Composition per liter Pancreatic digest of Phenol Carbohydrate pH at 25 C Source This medium is available as a premixed powder from BD Diagnostic Systems. Carbohydrate Solution Composition per Preparation of Carbohydrate Solution Add carbohydrate to distilled deionized water and bring volume to . Mix thoroughly. Filter sterilize. Preparation of Medium Add components except carbohydrate solution to distilled deionized water and bring volume to . Mix thoroughly. Adjust pH to if necessary. Autoclave for 15 min at 15 psi pressure-121 C. Cool to 45 -50 C. Aseptically add of sterile carbohydrate solution. Pour into sterile Petri dishes or distribute into sterile tubes. Allow tubes to cool in a slanted position. Use For the determination of fermentation reactions. Bacteria that can ferment the added carbohydrate turn the medium yellow. Phenol Red Agar Composition per liter Proteose peptone No. Beef Phenol Carbohydrate pH at 25 C Source This medium is available as a premixed powder from BD Diagnostic Systems. Carbohydrate Solution Composition per Preparation of Carbohydrate Solution Add carbohydrate to distilled deionized water and bring volume to . Mix thoroughly. Filter sterilize. Preparation of Medium Add components except carbohydrate solution to distilled deionized water and bring volume to . .
