Handbook of Microbiological Media, Fourth Edition part 149 is an invaluable reference for every medical, veterinary, diagnostic, and academic laboratory, and now in its fourth edition, it is even more complete. This edition carries on the tradition of CRC Press handbook excellence, listing the formulations, methods of preparation, and uses for more than 7000 microbiological media. With 1500 more entries than any previous edition, the handbook includes both classic and modern media used for the identification, cultivation, and maintenance of diverse bacteria, archaea, and fungi | R70-2 Broth Modified with Glucose 1475 Nicotinic Calcium p-Aminobenzoic Thioctic Folic Preparation of Wolfe s Vitamin Solution Add components to distilled deionized water and bring volume to . Mix thoroughly. Filter sterilize. 100X Modified Salts Composition per liter HCl Preparation of 100X Modified Salts Add components to dis-tilled deionized water and bring volume to . Mix thoroughly. Filter sterilize. Preparation of Medium Add components except Wolfe s vitamin solution and 100X modified salts to distilled deionized water and bring volume to . Mix thoroughly. Adjust pH to . Gently heat and bring to boiling. Autoclave for 15 min at 15 psi pressure-121 C. Cool to 45 -50 C. Aseptically add sterile Wolfe s vitamin solution and 100X modified salts. Mix thoroughly. Pour into sterile Petri dishes or distribute into sterile tubes. Use For the cultivation of Acetobacter xylinum. R70-2 Broth Modified with Fructose Composition per liter Yeast Dimethyl glutaric NH4 Trisodium Wolfe s vitamin 100X modified pH at 25 C Wolfe s Vitamin Solution Composition per liter Nicotinic Calcium p-Aminobenzoic Thioctic Folic Preparation of Wolfe s Vitamin Solution Add components to distilled deionized water and bring volume to . Mix thoroughly. Filter sterilize. 100X Modified Salts Composition per liter .
