Handbook of Microbiological Media, Fourth Edition part 152 is an invaluable reference for every medical, veterinary, diagnostic, and academic laboratory, and now in its fourth edition, it is even more complete. This edition carries on the tradition of CRC Press handbook excellence, listing the formulations, methods of preparation, and uses for more than 7000 microbiological media. With 1500 more entries than any previous edition, the handbook includes both classic and modern media used for the identification, cultivation, and maintenance of diverse bacteria, archaea, and fungi | Rhodospirillaceae Enrichment Medium 1505 Vitamin B12 Neutralized sulfide pH at 25 C Ferric Citrate Solution Composition per Ferric Preparation of Ferric Citrate Solution Add ferric citrate to dis-tilled deionized water and bring volume to . Mix thoroughly. Sparge under 100 N2 for 3 min. Autoclave for 15 min at 15 psi pres-sure-121 C. Store under N2. Trace Elements Solution SL-6 Composition per liter ZnSO4-7H2O. Na2MoO4-2H2O. Preparation of Trace Elements Solution SL-6 Add components to distilled deionized water and bring volume to . Mix thoroughly. Vitamin B12 Solution Composition per Vitamin Preparation of Vitamin B12 Solution Add vitamin B12 to distilled deionized water and bring volume to . Mix thoroughly. Sparge under 100 N2 for 3 min. Autoclave for 15 min at 15 psi pres-sure-121 C. Store under N2. Neutralized Sulfide Solution Composition per Preparation of Neutralized Sulfide Solution Add Na2S-9H2O to distilled deionized water in a 250mL screw-capped bottle fitted with a butyl rubber septum and bring volume to . Add a magnetic stir bar. Mix thoroughly. Sparge under 100 N2 for 3 min. Autoclave for 15 min at 15 psi pressure-121 C. Cool to room temperature. Adjust pH to about with sterile 2M H2SO4. Do not open the bottle to add H2SO4 use a sterile syringe. Stir the solution continuously to avoid precipitation of elemental sulfur. The final solution should be clear and yellow in color. Preparation of Medium Add components except neutralized sulfide solution to distilled deionized water and bring volume to . Mix thoroughly. Gently heat and bring to boiling. Boil for 3-4 min under a stream of 100 N2. Distribute of the prepared medium into screw-capped tubes that have been flushed with 100 N2. Autoclave for 15 min at 15 psi .
