Handbook of Microbiological Media, Fourth Edition part 161 is an invaluable reference for every medical, veterinary, diagnostic, and academic laboratory, and now in its fourth edition, it is even more complete. This edition carries on the tradition of CRC Press handbook excellence, listing the formulations, methods of preparation, and uses for more than 7000 microbiological media. With 1500 more entries than any previous edition, the handbook includes both classic and modern media used for the identification, cultivation, and maintenance of diverse bacteria, archaea, and fungi | SP 4 Medium 1595 Phenol Deoxyguanosine. Glutathione Nicotinamide adenine Tween Sodium Coenzyme Flavin adenine Nicotinamide adenine dinucleotide Uridine Choline p-Aminobenzoic D-Calcium Folic Preparation of CMRL 1066 10X with Glutamine Add components to distilled deionized water and bring volume to . Mix thoroughly. Adjust pH to . Filter sterilize. Fresh Yeast Extract Solution Composition per Baker s yeast live pressed starch-free . Preparation of Fresh Yeast Extract Solution Add the live Baker s yeast to of distilled deionized water. Autoclave for 90 min at 15 psi pressure-121 C. Allow to stand. Remove supernatant solution. Adjust pH to . Penicillin Solution Composition per Penicillin G. 1 000 000U Preparation of Penicillin Solution Add penicillin G to distilled deionized water and bring volume to . Mix thoroughly. Filter sterilize. Preparation of Medium To of cooled sterile base solution aseptically add of sterile inactivated fetal calf serum of sterile yeast extract of sterile CMRL 1066 10X with glutamine of sterile fresh yeast extract solution of Phenol Red solution and of sterile penicillin solution. Mix thoroughly. Aseptically distribute into sterile tubes. Allow tubes to cool in a slanted position. Use For the cultivation of tick-derived Mycoplasma
