Handbook of Microbiological Media, Fourth Edition part 173 is an invaluable reference for every medical, veterinary, diagnostic, and academic laboratory, and now in its fourth edition, it is even more complete. This edition carries on the tradition of CRC Press handbook excellence, listing the formulations, methods of preparation, and uses for more than 7000 microbiological media. With 1500 more entries than any previous edition, the handbook includes both classic and modern media used for the identification, cultivation, and maintenance of diverse bacteria, archaea, and fungi | Thermoanaerobacter tengcongensis Medium 1715 Glucose Na2S-9H2O Vitamin Yeast extract Trace elements solution pH at 25 C Vitamin Solution Composition per liter Nicotinic p-Aminobenzoic Lipoic Folic Vitamin B12 . Preparation of Vitamin Solution Add components to distilled deionized water and bring volume to . Mix thoroughly. Sparge with 80 H2 20 CO2. Filter sterilize. Glucose Solution Composition per Preparation of Glucose Solution Add glucose to distilled deion-ized water and bring volume to . Mix thoroughly. Filter sterilize. Yeast Extract Solution Composition per Yeast Preparation of Yeast Extract Solution Add yeast extract to dis-tilled deionized water and bring volume to . Mix thoroughly. Autoclave under 100 N2 for 15 min at 15 psi pressure-121 C. Cool to room temperature. Trace Elements Solution SL-10 Composition per liter HCl 25 solution . Preparation of Trace Elements Solution SL-10 Add FeCl24H2O to of HCl solution. Mix thoroughly. Add distilled deionized water and bring volume to . Add remaining components. Mix thoroughly. Sparge with 80 N2 20 CO2. Autoclave for 15 min at 15 psi pressure-121 C. Na2S-9H2O Solution Composition per Preparation of Na2S-9H2O Solution Add Na2S-9H2O to dis-tilled deionized water and bring volume to . Mix thoroughly. Autoclave under 100 N2 for 15 min at 15 psi pressure-121 C. Cool to room temperature. Preparation of Medium Prepare and dispense medium under 80 N2 20 CO2 gas mixture. Add components except .
