Handbook of Microbiological Media, Fourth Edition part 174 is an invaluable reference for every medical, veterinary, diagnostic, and academic laboratory, and now in its fourth edition, it is even more complete. This edition carries on the tradition of CRC Press handbook excellence, listing the formulations, methods of preparation, and uses for more than 7000 microbiological media. With 1500 more entries than any previous edition, the handbook includes both classic and modern media used for the identification, cultivation, and maintenance of diverse bacteria, archaea, and fungi | Thermodesulfotobacterium Agar 1725 Na2S-9H2O Na2S2O4 Trace elements solution NaHCO3 pH at 25 C NaHCO3 Solution Composition per Preparation of NaHCO3 Solution Add NaHCO3 to distilled de-ionized water and bring volume to . Mix thoroughly. Sparge with 80 N2 20 CO2. Autoclave for 15 min at 15 psi pressure-121 C. Na2S-9H2O Solution Composition per Preparation of Na2S-9H2O Solution Add Na2S-9H2O to dis-tilled deionized water and bring volume to . Mix thoroughly. Sparge with 100 N2. Autoclave for 15 min at 15 psi pressure-121 C. Na2S2O3 Solution Composition per Preparation of Na2S2O3 Solution Add Na2S2O3-5H2O to dis-tilled deionized water and bring volume to . Mix thoroughly. Sparge with 100 N2. Autoclave for 15 min at 15 psi pressure-121 C. Trace Elements Solution SL-10 Composition per liter HCl 25 solution . Preparation of Trace Elements Solution SL-10 Add FeCl2-4H2O to of HCl solution. Mix thoroughly. Add distilled deionized water and bring volume to . Add remaining components. Mix thoroughly. Preparation of Medium Prepare and dispense medium under 100 N2. Add components except Na2S-9H2O solution Na2S2O3 solution and NaHCO3 solution to distilled deionized water and bring volume to . Mix thoroughly. Gently heat and bring to boiling. Cool to room temperature while sparging with 100 N2. Anaerobically distribute volumes into tubes. Autoclave for 15 min at 15 psi pres-sure-121 C. Aseptically and anaerobically add of sterile Na2S-9H2O solution and of sterile Na2S2O4 solution to each tube. Aseptically and anaerobically add a sufficient volume of sterile NaHCO3 solution to each tube to bring the pH to . Use For the cultivation of Thermodesulforhabdus .
