Handbook of Microbiological Media, Fourth Edition part 175 is an invaluable reference for every medical, veterinary, diagnostic, and academic laboratory, and now in its fourth edition, it is even more complete. This edition carries on the tradition of CRC Press handbook excellence, listing the formulations, methods of preparation, and uses for more than 7000 microbiological media. With 1500 more entries than any previous edition, the handbook includes both classic and modern media used for the identification, cultivation, and maintenance of diverse bacteria, archaea, and fungi | Thermosipho africanus Medium 1735 Glucose Solution Composition per Preparation of Glucose Solution Add glucose to distilled deion-ized water and bring volume to . Mix thoroughly. Filter sterilize. Yeast Extract Solution Composition per Yeast Preparation of Yeast Extract Solution Add yeast extract to dis-tilled deionized water and bring volume to . Mix thoroughly. Filter sterilize. Preparation of Medium Add components except glucose solution and yeast extract solution to distilled deionized water and bring volume to . Mix thoroughly. Adjust pH to with 10A H2SO4. Autoclave for 15 min at 15 psi pressure-121 C. Aseptically add of sterile glucose solution and of sterile yeast extract solution. Mix thoroughly. Aseptically distribute into sterile tubes or flasks. Final pH should be . Use For the aerobic cultivation and maintenance of Thermoplasma volcanium. Thermoproteus Medium Composition per liter Solution Solution Solution pH at 25 C Solution A Composition per NH4 Yeast Trace Preparation of Solution A Add components to distilled deionized water and bring volume to . Mix thoroughly. Immediately filter sterilize. Trace Elements Composition per liter Preparation of Trace Elements Add components to distilled de-ionized water and bring volume to . Mix thoroughly. Adjust pH to with H2SO4 to retard precipitation. Solution B Composition per Preparation of Solution B Add sulfur to of distilled deionized water. Autoclave for 30 min at 0 psi pressure-100 C on 3 consecutive days. Solution C Composition per Preparation of Solution C
