Handbook of Microbiological Media, Fourth Edition part 180 is an invaluable reference for every medical, veterinary, diagnostic, and academic laboratory, and now in its fourth edition, it is even more complete. This edition carries on the tradition of CRC Press handbook excellence, listing the formulations, methods of preparation, and uses for more than 7000 microbiological media. With 1500 more entries than any previous edition, the handbook includes both classic and modern media used for the identification, cultivation, and maintenance of diverse bacteria, archaea, and fungi | Thiosulfate Salts Broth 1785 NH4 6MO7O24 4H2O. Preparation of Vishniac and Santer Trace Metals Add components to distilled deionized water and bring volume to . Adjust pH to with KOH. Mix thoroughly. Preparation of Medium Add components to distilled deionized water and bring volume to . Mix thoroughly. Adjust pH to . Filter sterilize. Aseptically distribute into sterile tubes or flasks. Use For the cultivation and maintenance of Thiosphaera pantotro-pha. Thiosphaerapantotropha Medium Composition per Yeast extract Trace elements solution pH at 25 C Yeast Extract Solution Composition per Yeast Preparation of Yeast Extract Solution Add yeast extract to distilled deionized water and bring volume to . Mix thoroughly. Autoclave for 15 min at 15 psi pressure-121 C. Trace Elements Solution SL-10 Composition per liter HCl 25 solution . Preparation of Trace Elements Solution SL-10 Add FeCl24H2O to of HCl solution. Mix thoroughly. Add distilled deionized water and bring volume to . Add remaining components. Mix thoroughly. Sparge with 100 N2. Autoclave for 15 min at 15 psi pressure-121 C. Preparation of Medium Add components except yeast extract solution to distilled deionized water and bring volume to . Mix thoroughly. Gently heat and bring to boiling. Autoclave for 15 min at 15 psi pressure-121 C. Cool to 50 -55 C. Aseptically add of sterile yeast extract solution. Mix thoroughly. Pour into sterile Petri dishes or distribute into sterile tubes. Use For the cultivation and maintenance of Paracoccus denitrificans. Thiosulfate Citrate Bile Salts Sucrose Agar See TCBS Agar Thiosulfate-Oxidizing Medium .
