Handbook of Microbiological Media, Fourth Edition part 181 is an invaluable reference for every medical, veterinary, diagnostic, and academic laboratory, and now in its fourth edition, it is even more complete. This edition carries on the tradition of CRC Press handbook excellence, listing the formulations, methods of preparation, and uses for more than 7000 microbiological media. With 1500 more entries than any previous edition, the handbook includes both classic and modern media used for the identification, cultivation, and maintenance of diverse bacteria, archaea, and fungi | TMA Mineral Medium 1795 Glutamine CaCl2-2H2O solution optional . pH at 25 C Glutamine Solution Composition per NaCl solution . Preparation of Glutamine Solution Add the glutamine to the NaCl solution. Mix thoroughly. Filter sterilize. Preparation of Medium Add components except glutamine and serum to distilled deionized water and bring volume to . Mix thoroughly. Adjust pH to with 10 Na2CO3 solution. Filter sterilize. Aseptically add of sterile glutamine solution and of sterile serum. Human serum bovine serum horse serum or fetal calf serum may be used. Mix thoroughly. To grow cells in a monolayer aseptically add of a sterile 10 CaCl2-2H2O solution. To grow cells in suspension omit the CaCl2-2H2O solution. Use For preparation of Eagle s minimal medium for the cultivation of cells in monolayer or suspension in tissue culture. Tissue Culture Minimal Medium Eagle Spinner Modified TC Minimal Medium Eagle Spinner Modified MEM-S Composition per Phenol Calcium Choline Folic Glutamine pH at 25 C Glutamine Solution Composition per L-Glutamine . NaCl solution . Preparation of Glutamine Solution Add the glutamine to the NaCl solution. Mix thoroughly. Filter sterilize. Preparation of Medium Add components except glutamine and serum to distilled deionized water and bring volume to . Mix thoroughly. Adjust pH to .
