Handbook of Microbiological Media, Fourth Edition part 188 is an invaluable reference for every medical, veterinary, diagnostic, and academic laboratory, and now in its fourth edition, it is even more complete. This edition carries on the tradition of CRC Press handbook excellence, listing the formulations, methods of preparation, and uses for more than 7000 microbiological media. With 1500 more entries than any previous edition, the handbook includes both classic and modern media used for the identification, cultivation, and maintenance of diverse bacteria, archaea, and fungi | U9B Broth 1865 Preparation of Medium To of cooled sterile base agar aseptically add of sterile yeast dialysate of horse serum of sterile penicillin solution of sterile MES buffer solution of sterile Na2SO3 solution and of sterile urea solution. Mix thoroughly. Pour into 10mm x 35mm Petri dishes in volumes. Allow plates to stand overnight at 25 C to remove excess surface moisture. Use within 48 hr. Use For the isolation and cultivation of Ureaplasma urealyticum. U9 Broth Urease Color Test Medium Composition per U9 Horse serum Penicillin G Urea Phenol Red pH at 25 C U9 Base Composition per Pancreatic digest of Papaic digest of soybean Preparation of U9 Base Add components to distilled deionized water and bring volume to . Mix thoroughly. Adjust pH to with 1jV HCl. Autoclave for 15 min at 15 psi pressure-121 C. Cool to 45 -50 C. Penicillin G Solution Composition per Penicillin Preparation of Penicillin G Solution Add penicillin G to dis-tilled deionized water and bring volume to . Mix thoroughly. Filter sterilize. Urea Solution Composition per Preparation of Urea Solution Add urea to distilled deionized water and bring volume to . Mix thoroughly. Filter sterilize. Phenol Red Solution Composition per Phenol Preparation of Phenol Red Solution Add Phenol Red to dis-tilled deionized water and bring volume to . Mix thoroughly. Filter sterilize. Preparation of Medium To of cooled sterile U9 base aseptically add of sterile horse serum of sterile penicillin G solution of sterile urea solution and of sterile Phenol Red solution. Mix thoroughly. Aseptically distribute into sterile tubes or flasks. Use For the isolation and identification of T-strain
