Handbook of Microbiological Media, Fourth Edition part 191 is an invaluable reference for every medical, veterinary, diagnostic, and academic laboratory, and now in its fourth edition, it is even more complete. This edition carries on the tradition of CRC Press handbook excellence, listing the formulations, methods of preparation, and uses for more than 7000 microbiological media. With 1500 more entries than any previous edition, the handbook includes both classic and modern media used for the identification, cultivation, and maintenance of diverse bacteria, archaea, and fungi | Vulcanibacillus Medium 1895 Use For the isolation of vancomycin resistant enterococci VRE from clinical samples. Nonresistant enterococci containing the Van C genes will not grow on this selective supplement suppresses growth of Gram-negative bacteria and E. gallinarum. The medium contains an indicator system to detect the growth of esculin-hydrolyzing organisms. Enterococci produce black zones around the colonies from the formation of black iron phenolic compounds derived from esculin-hydrolyis products and ferrous iron. VRE Agar Composition per Yeast Sodium Ferric ammonium g Selective supplement pH at 25 C Source This medium is available as a premixed powder from Oxoid Unipath. Selective Supplement Solution Composition per Preparation of Selective Supplement Solution Add gentamicin to distilled deionized water and bring volume to . Mix thoroughly. Filter sterilize. Preparation of Medium Add components except selective supplement solution to distilled deionized water and bring volume to . Mix thoroughly. Gently heat while stirring and bring to boiling. Autoclave for 15 min at 15 psi pressure-121 C. Cool to 50 C. Asep-tially add selective supplement solution. Mix thoroughly . Pour into sterile Petri dishes. Use For the isolation of high-level aminoglycoside-resistant enterococci HLARE from clinical samples. Nonresistant enterococci containing the Van C genes will not grow on this selective supplement suppresses growth of Gram-negative bacteria and E. gallinarum. The medium contains an indicator system to detect the growth of esculin-hydrolyzing organisms. Enterococci produce black zones around the colonies from the formation of black iron phenolic compounds derived from esculin hydrolyis products and ferrous iron. VRE Broth Composition per Calf brain infusion .
