Introduction to Modern Liquid Chromatography, Third Edition part 2. High-performance liquid chromatography (HPLC) is today the leading technique for chemical analysis and related applications, with an ability to separate, analyze, and/or purify virtually any sample. Snyder and Kirkland's Introduction to Modern Liquid Chromatography has long represented the premier reference to HPLC. This Third Edition, with John Dolan as added coauthor, addresses important improvements in columns and equipment, as well as major advances in our understanding of HPLC separation, our ability to solve problems that were troublesome in the past, and the application of HPLC for new kinds of samples. . | viii CONTENTS Gradient Systems 112 Special Applications 112 Low-Flow Micro and Nano Applications 112 High-Flow Prep Applications ii3 HigA-PressureApplications 113 Autosamplers 113 Six-Port Injection Valves .14 Filted-Looplnjection sis Partial-LoopMjretmi 115 Autosamp terDee . gnSi 116 Pull-to-Fi ll Autosamplers 117 Pusheto-FljjAuposamplecs 118 Autosam p i . ui. Samp-e-SizeEffecsSiiis Imjection pzlumu lpo tejnction pzlneet ilp Other 122 Column . FractionCollesUeis -23 DivprsjeA 124 Column Ovens 125 T3md4ratul n-Control peeujrements t25 Oven Desi4 . . .p6 fitter Heater 126 DataSystem Leper-m entalAids i27 System Centro-. .9 Data 9 Data Procestmg. 13P RrportGeneration 130 Regutatory nctis-s P30 Extra-Colume 8ffncts rjs Maintenance System-PirformAussTpsts 53i MAtallstfstQApUffcation O retional QuAp6caltion and Peesormac5c Qualification 132 Gcadient Performance Test 132 Atlditipsa1 S tem Csscks 5a5 CONTENTS ÎX Preventive Maintenance 138 Periodic Maintenance 138 Suggestions for Routine Applications 141 Repairs 143 Personnel 143 Record Keeping 143 Redite R f ir Recommendarions 144 References 144 4 DETECTION Introduction 148 Detector Characteristics 149 General Layout 149 Detection Techniques 151 Bulk Property Detectors 151 Sample-Specific Detectors 152 Mobile-PhasnModification H hnsattd diP ues 152 Signal aid Assay Precision Noioeand N 155 Detection .oiitr 157 Linoarity i58 Introduction to IndividralDetectoss 160 .
