Introduction to Modern Liquid Chromatography, Third Edition part 72. High-performance liquid chromatography (HPLC) is today the leading technique for chemical analysis and related applications, with an ability to separate, analyze, and/or purify virtually any sample. Snyder and Kirkland's Introduction to Modern Liquid Chromatography has long represented the premier reference to HPLC. This Third Edition, with John Dolan as added coauthor, addresses important improvements in columns and equipment, as well as major advances in our understanding of HPLC separation, our ability to solve problems that were troublesome in the past, and the application of HPLC for new kinds of samples. . | 666 ENANTIOMER SEPARATIONS Thermodynamics of Direct Chromatographic Enantiomer Separation 716 Site-Selective Thermodynamics 717 INTRODUCTION Previous chapters have described HPEC procedures of wide applicability that is separations that can be used for many different kinds of samples. Usually method development can be started with any of various columns or conditions and resolution is then systematically improved by varying separation conditions. Consequently there are often many different ways of successfully separating a particular sample. Suchan approach cannotbeusedfor enantiomers however which require highly specfoOecdaediniquecanO mmott cuces the selectiooofthncolomncs tie ceiticel ceep utOerea saipativ colomniseelecieO subsequent ehanuts mother tonditions art tmtkelyfo bc enantiomero cninostre lodanprrftrmed rootivciy in many seicatch andcoutine laboratories andeae oh cteor n rt ncei iuthephvemaecutlcallnduateirf. Varioas technologieiand tookhave beandeveloped Otatqravidearich tooiboxtneeparvtt virtually ano sample. Amongehpavallenie took dioect separation wifi a cHinai stationary pUufs CSP aneounsioselcetiaeor chirala cviumn tarbucomu vPs predominant lmdmostbcaopted asocedure Outovetneon a proctdvre .Howdc ee ihe identificatiou bl the mcst sctteALe CqP mobile-nOase camhlnationfor qpvoticltlar analyte canbeehellengino. Aue iotOefpvcifictfyofmolceulbe-recoonisinnfareacP combination ccCSV h hidaUelectorlahá 1 las sunsitivity to minoestroc-tural variationtintitherfhnsoluiv ovCqP reSiable bredictionsofanappropciett column free ahqlytemoleeu1ac ed hctnreaecocyet hardly 1088 . Dátanoste suches Vhirdase Pttp prnuidetselv iathe selection oSqolumqauO sicrting mobilcpAasao dooedon avalyts 01 1 0 1 . A databaseapproach willbn of greavevi valuewqvnphe eqvntiomevt at interesa are included indie aasubvso. nObs pt i i tdica n beusetul for enuntiomect of relatedstencsvees .
