Introduction to Modern Liquid Chromatography, Third Edition part 88. High-performance liquid chromatography (HPLC) is today the leading technique for chemical analysis and related applications, with an ability to separate, analyze, and/or purify virtually any sample. Snyder and Kirkland's Introduction to Modern Liquid Chromatography has long represented the premier reference to HPLC. This Third Edition, with John Dolan as added coauthor, addresses important improvements in columns and equipment, as well as major advances in our understanding of HPLC separation, our ability to solve problems that were troublesome in the past, and the application of HPLC for new kinds of samples. . | 826 TROUBLESHOOTING Murcof rhe eetm canresulrinleaksarrhern non valve. standard h-poet iu eetion valve uses arotatma turnsagarnar a stationary. stator to which the tube connections are made. The rotor typically is made of a hard polymer such as PEEK Vespel or one of the fluoropolymers and contains tiny passages that connect the fluid passages in the stator. The stator may consist of the stamleaa-tseelvalvebody orit mayba aninsertlathavalvemtdtaf ceramic on aarthrr hard smooterurface. hatha thetaotea comprises tnepmrtian of the valveimide theairclr whetrns ale sartor iatht mart immediatehrorn sda injection valves We rotor is adat disk with tdrta aidntn-shapadgroodestrsFe aurfaee simitar tothat shottn inthn sketch of Figuea If-ta. Thetegtoovas laecupwith the euOt oS t r etbecomieationr on the stator shown at are aaaOanhirdas in Otvura ty-SFaSuone foairion . load the duw ehannels thatresuSt avnnecs pnnr M vnd5o6 as teen in Figure thteotov ieaotated ra i tdrinjeea oosition iadidlerndt set of port is cv neiaeV in Oavenampir 2ddi4-a and i -l. If a m a pisceofhotdmaieekl rucdvt avarticle afaolumn-paakmeor a bit of staidleslstadt fromavaoriy aut robejgetacaugat inone ofthe j anavesot the injectot it een farma connecting passageOetween two of thagrovvot rh showa beswtdn pond 4anh5 ev result is cnvst-Scai eet FdrlWdara auidfvom onv hydrauliaportion o. the system leaks into anotSer. This aao showmp asflvadleaktag num sOa injectioner wasre port as a proaSemot vreeislon dus to Cddad leekine intooa ornia tla dampSe lavp or sometimes aBacan -ovra neiOitemlaei tion .Rhtorc5eal enapcrtiblo stator repkma ei l v ilac taqait dto correatausproUlam. Alsa breureto cleaa the remainVar oftheoalvetOoroughly tvrernoveasa parlitvktematter. Moreanmia nmlatel i tm- 5eal wiild U es anesult ofonrmalrriclivnalwetr. The rotor sdals arm Calignhh .
