Introduction to Modern Liquid Chromatography, Third Edition part 94. High-performance liquid chromatography (HPLC) is today the leading technique for chemical analysis and related applications, with an ability to separate, analyze, and/or purify virtually any sample. Snyder and Kirkland's Introduction to Modern Liquid Chromatography has long represented the premier reference to HPLC. This Third Edition, with John Dolan as added coauthor, addresses important improvements in columns and equipment, as well as major advances in our understanding of HPLC separation, our ability to solve problems that were troublesome in the past, and the application of HPLC for new kinds of samples. . | 886 PROPERTIES OF HPLC SOLVENTS are rarely ingested rather their primary effect is by contact or inhalation. Rubbing alcohol z-propanol with an LD50 of 400 is clearly not a problem in terms of either contact or inhalation. Material Safety Data Sheets MSDS should be consulted before handling any solvent or reagent. REFERENCES 1. J. 10 1982 856. 2. High-Purity Solvent Guide Burdick Jackson Laboratories Muskegon MI 1980. 3. J. A. Riddick Bungee Wey I e ee New Y k 1970. 4. L. R. Snyder in High-performance Liquid Chromatography Advances and Perspectives Vol. 3 C. Horvath ed. Academic Press New York 1983 p. 157. 5. K. Valko L. R. Snyder and J. . Chromatogr. 656 1993 501. 6. L. R. Snader . 16 1978 223. 7. H. Colin G. GuiocRon T Czjjkows0a rnd I. MiedziaC . Chromatogr. 167 1978 11. 8. M. A. Quarry R L. Groh and . J. Chromotogr 285 1984 1. 9. J. Billei K. P rar cl horcii G LiiGens K. Choiknet Gj Rozrng aod G. Desmet J. Chromatsgr. A 181Q i2008 G0. APPENDIX II PREPARING BUFFERED MOBILE PHASES II. 1 SEQUENCE OF OPERATIONS Buffered mobile phases can be prepared by the following sequence of operations 1. combine the buffer ingredients with water to obtain the aqueous buffer . 1 A __. . 2. confirm or adjust the pH of solution A with a pH meter 3. combine a given volume . 200nJL oforgiuiic buffer solution B with a given volume . 800 mL of solution A from step 2 to obtain the final mobile phncg 2g oegembbuffenm egis ccaniplc 4. check the pH of the final mobile phase optional Because a pH mencgremenu for a moglie phasethatcociarns orgernnbuSSec is unreliable due to UrsftoO tSegH mster shep4 ss bor deteotrng mejur errors in the formulaiifnoocompehing Swurolutinno wlthrhesemo organic content. Most laboratories elect to skip step 4. The usual appcoachi hi otepl 0 toforrnokte oiiueousbufierr of differing pH Al and A2 oeh ehon cgmUsoo gore iwo eolgiiuns iothe coerectgruportinsutn obtain finolsoiufion A .
