Tham khảo tài liệu 'atomic force microscopy episode 2 part 6', kỹ thuật - công nghệ, cơ khí - chế tạo máy phục vụ nhu cầu học tập, nghiên cứu và làm việc hiệu quả | 308 Miyazaki and Hayashi First the sensitivity of the AFM system is determined using a cantilever for each measurement and a glass cover slip. Immediately before AFM observation strip specimens are cut out from the artery. Each specimen is fixed on silicone rubber with the endothelial side up using pins at its in vivo axial and circumferential lengths. The specimen attached to the rubber is covered with HBSS of room temperature and is mounted onto the sample stage of the AFM. After the surface topograph of the endothelium is determined force curves are obtained at various locations in each endothelial cell. Force-indentation relations are determined from the force curves. Stiffness is calculated from the force-indentation relations. 2. Materials 1. Living animal. 2. Pentobarbital sodium solution. 3. Gentian violet solution dissolve gentian violet pellets in distilled water to make saturated solution store the solution in a refrigerator at 4 C see Note 1 . 4. HBSS mix mM NaCl mM KCl mM Na2HPO4 12 H2O mM KH2PO4 mM MgCl2 6H2O mM MgSO4 7H2O mM CaCl2 mM NaHCO3 and mM dextrose in distilled water filtrate with a membrane filter having the pore size of pm adjust the pH to and store in a refrigerator at 4 C see Note 2 . 5. Silicone rubber cut a 3-mm thick silicone rubber sheet into appropriate size . 5 X 5 mm . Ultrasonically wash it with acetone ethanol and then distilled water for 10 min for each and dry it. 6. Stainless steel pin cut an approximately diameter stainless-steel wire into short pieces each having the length of about 4 mm and bend one end of each to an angle of about 90 to make an L-shape. Ultrasonically wash them in the same way as that for the silicone rubber and dry see Note 3 . 3. Methods . Resection of Arterial Segment . Exposure of Artery Under Anesthesia 1. Induce general anesthesia to an animal by the injection of pentobarbital sodium into the vein or the abdominal cavity see Note 4 . 2.
