Tuyển tập các báo cáo nghiên cứu về sinh học được đăng trên tạp chí sinh học Journal of Biology đề tài: Functional genomics of cell morphology using RNA interference: pick your style, broad or deep. | J. Biol. Journal of Biology BioMed Central Minireview Functional genomics of cell morphology using RNA interference pick your style broad or deep Thomas D Pollard Address Departments of Molecular Cellular and Developmental Biology and Cell Biology Yale University New Haven CT 06520 USA. Email Published 1 October 2003 Journal of Biology 2003 2 25 The electronic version of this article is the complete one and can be found online at http content 2 4 25 2003 BioMed Central Ltd Abstract Several new studies have used RNA interference to screen for protein functions affecting cell shape mitosis and cytokinesis of Drosophila cells in culture. One broad survey of nearly 1 000 proteins and three studies focused on cytoskeletal and motor proteins have identified key proteins essential for these processes in animal cells. Given complete genome sequences from a growing number of organisms investigators are confronted with how most efficiently to complete the inventory of proteins that participate in complex cellular processes such as cytokinesis cellular motility or the establishment of asymmetric cell shapes. Such an inventory is the essential first step in beginning to think mechanistically about how any such system of interdependent parts functions as a whole. In the pre-genomic era classical forward genetics screening of mutants biochemical isolation with reconstitution and pharmacology provided laborious but definitive methods to connect genes with molecular functions working on one protein at a time. Now strategies can aim for broad or even complete coverage. Experiments with budding yeast led the way with four sorts of experiments first a complete set of deletion mutations which showed that only 19 of the 6 200 genes are required for viability in the laboratory 1 second crosses between viable deletion strains revealing synthetic interactions 2 third large-scale two-hybrid assays mapping out networks of protein-protein interactions 3 and
