Tuyển tập báo cáo nghiên cứu khoa học của trường đại học nông nghiệp 1 đề tài: A canine distemper virus isolated from an autopsied dog in Hanoi, Vietnam. | Journal of Science and Development April 2008 70-74 HANOI UNIVERSITY OF AGRICULTURE A CANINE DISTEMPER VIRUS ISOLATED FROM AN AUTOPSIED DOG IN HANOI VIETNAM Nguyen Thi Lan Yamaguchi Ryoji Nguyen Huu Nam Trần Trung Kiên Faculty of Veterinary Medicine FVM Ha noi University of Agriculture HUA Department ofVeterinary Phathology Faculty of Agriculture University of Miyazaki Miyazaki 889-2192 Japan ABSTRACT The fresh samples of lung taken post mortem from an autopsied dog with evidence of canine distemper was used for isolation of canine distemper virus CDV . The lung of the dog was congested by hemorrhagic and pneumonic with much fluid or exudate. Severe interstitial pneumonia was found with some eosinophilic cytoplasmic inclusion bodies in alveolar epithelium and CDV antigens strongly positive among alveoli. The CDV was isolated by using Vero cells with CDV receptor and was confirmed by RT-PCR of the 429 bp fragment P gene. The isolated strain CDV-HN1 had the titer of TCID50 25 pl. The results indicated that the dog population in Hanoi was infected with CDV. Key words Canine distemper dog isolation Hanoi Vietnam. 1. INTRODUCTION Canine distemper disease is a fatal disease in dogs ferrets and wild carnivores cause by Canine distemper virus CDV . The first target of CDV is lymphoid tissues causing immunosuppression later the virus spreads to epithelial and nervous tissues respectively. CDV is a member of genus Mobillivirus in the Paramyxoviridae family and closely related to measle virus and Phocine distemper virus PDV . The Mobillivirus is composed of 6 protein genes. Fusion F and Hemagglutinin H glycoprotein are envelope proteins. H protein is a receptor binding site and F protein induces the fusion between viral envelope and host cell membrane. Nucleocapsid protein N Phosphoprotein P and Large protein L are a nucleocapsid core cooperating in viral replication. Matrix M protein assembles between the envelope proteins and the nucleocapsid core Griffin and .
