Tuyển tập các báo cáo nghiên cứu về sinh học được đăng trên tạp chí sinh học Journal of Biology đề tài: Mapping the protistan ‘rare biosphere’. | Journal of Biology Minireview Mapping the protistan rare biosphere Scott C Dawson and Kari D Hagen Addresses Department of Microbiology University of California Davis One Shields Avenue Davis CA 95616 USA. Correspondence Scott C Dawson. Email scdawson@ Abstract The use of cultivation-independent approaches to map microbial diversity including recent work published in BMC Biology has now shown that protists like bacteria archaea are much more diverse than had been realized. Uncovering eukaryotic diversity may now be limited not by access to samples or cost but rather by the availability of full-length reference sequence data. See research article http 1741-7007 7 72 For several decades now microbiologists have lived with the growing realization that the majority of extant microbes are not in our culture collections. In fact our historical reliance on cultivation to identify and quantify microbes has resulted in our missing upwards of 95 of extant bacterial and archaeal diversity. Using cultivationindependent molecular approaches to identify microbes by genetic sequence - specifically small subunit ribosomal RNA SSU rRNA sequences - we have begun to map the true microbial diversity of the Earth. This cultivationindependent approach to identifying diversity has recently benefited from the development of next-generation sequencing technology and a concomitant drop in sequencing costs. With respect to molecular surveys of microbial diversity in natural environmental samples pyrosequencing approaches provide unprecedented sampling depth. Such deep sequencing has purportedly uncovered a rare and extensive biosphere of bacteria and archaea with a diversity that is perhaps several orders of magnitude greater than we had anticipated 1 . And although there may have been initial overestimations of the magnitude of the rare biosphere because of the intrinsic sequence error rate pyrosequencing produces 2 many rare and novel microbes are still .
