Tuyển tập các báo cáo nghiên cứu về sinh học được đăng trên tạp chí sinh học Journal of Biology đề tài: Apical polarity in three-dimensional culture systems: where to now? | Inman and Bissell Journal of Biology 2010 9 2 http content 9 1 2 Journal of Biology MINIREVIEW L__ Apical polarity in three-dimensional culture systems where to now Jamie L Inman and Mina J Bissell See research article http 1741-7007 7 77 Abstract Delineation of the mechanisms that establish and maintain the polarity of epithelial tissues is essential to understanding morphogenesis tissue specificity and cancer. Three-dimensional culture assays provide a useful platform for dissecting these processes but as discussed in a recent study in BMC Biology on the culture of mammary gland epithelial cells multiple parameters that influence the model must be taken into account. The cell in culture indeed is an adaptable organism. Unless conditions are strictly defined the answers will have little relevance to the questions asked. .There is one point however that cannot be emphasized enough While it is true that the usefulness of a culture system is increased by how far it is developed to mimic the in vivo situation we use cultured cells because the in vivo events in fact are not well understood. . We use cultured cells because we can simplify the milieu to understand normal physiology. We also use them to learn how to manipulate gene expression. While the molecular biologists reorder the genes the cell and developmental biologists by defining the cellular micro environment may call the shots in the long run 1 . Nearly 30 years later it is time to ask where to now Context in modeling epithelial tissues Control of context - defined here as the microenvironment and architecture of a cell culture - is essential to both the design and the interpretation of experiments performed in three-dimensional 3D culture. In these cultures multiple microenvironmental parameters such as cellular and tissue stiffness composition of the extracellular matrix ECM and media a substitute for lymph and blood and cell-cell interactions operate as they do in .
