Tuyển tập các báo cáo nghiên cứu về sinh học được đăng trên tạp chí sinh học Journal of Biology đề tài: No better time to FRET: shedding light on host pathogen interactions. | Hayward et al. Journal of Biology 2010 9 12 http content 9 2 12 Journal of Biology MINIREVIEW L__ No better time to FRET shedding light on host pathogen interactions Richard D Hayward 1 Jon D Goguen2 and John M Leong2 See research article http 1741-7007 7 81 Abstract Understanding the spatio-temporal subversion of host cell signaling by bacterial virulence factors is key to combating infectious diseases. Following a recent study by Buntru and co-workers published in BMC Biology we review how fluorescence Forster resonance energy transfer FRET has been applied to studying host-pathogen interactions and consider the prospects for its future application. Pathogen-induced signaling in mammalian cells The incidence of diseases caused by pathogenic bacteria is once again increasing with the resurgence of tuberculosis the rise of nosocomial infections and escalating resistance to antibiotics. Over the last two decades it has become increasingly apparent that many bacterial pathogens of substantial medical relevance engage in complex cross-talk with cells of their mammalian hosts. It is thus critical to decipher the molecular mechanisms of the underlying bacterial virulence weaponry which includes not only the secreted exotoxins like those that cause anthrax and cholera but also the multiple effectors injected directly into eukaryotic target cells via specialized nanomachines by pathogens like Escherichia coli O157 H7 and Yersinia. Such studies have already yielded new insights into the molecular basis of microbial pathogenesis suggesting new avenues for the development of novel diagnostics therapeutics and vaccines. These toxins and effectors can also be exploited as reagents to probe the pathways controlling key cellular processes such as signal Correspondence institute of Structural and Molecular Biology University College London and Birkbeck University of London Gower Street London WC1E 6BT UK .
