Tuyển tập các báo cáo nghiên cứu khoa học về toán học trên tạp chí toán học quốc tế đề tài: Veterinary Science. | J. Vet. Sci. 2009 10 1 15-22 DOI JOURNAL OF Veterinary Science Effects of L-NAME a non-specific nitric oxide synthase inhibitor on AlCl3-induced toxicity in the rat forebrain cortex r1 1 -2 1 r3 Ivana D. Stevanovic Marina D. Jovanovic Ankica Jelenkovic Miodrag Colic Ivana Stojanovic Milica Ninkovic1 Military Medical Academy Institute for Medical Research Crnotravska 17 Belgrade Serbia 2Institute for Biological Research Belgrade Serbia 3Department of Biochemistry Faculty of Medicine University of Nis Nis Serbia The present experiments were done to determine the effectiveness of a non-specific nitric oxide synthase inhibitor N-nitro-L-arginine methyl ester L-NAME on oxidative stress parameters induced by aluminium chloride AlClj intrahippocampal injections in Wistar rats. Animals were sacrificed 3 h and 30 d after treatments heads were immediately frozen in liquid nitrogen and forebrain cortices were removed. Crude mitochondrial fraction preparations of forebrain cortices were used for the biochemical analyses nitrite levels superoxide production malondialdehyde concentrations superoxide dismutase SOD activities and reduced glutathione contents. AlCl3 injection resulted in increased nitrite concentrations superoxide anion production malondialdehyde concentrations and reduced glutathione contents in the forebrain cortex suggesting that AlCl3 exposure promoted oxidative stress in this brain structure. The biochemical changes observed in neuronal tissues showed that aluminium acted as a pro-oxidant. However the nonspecific nitric oxide synthase NOS inhibitor L-NAME exerted anti-oxidant actions in AlCl3-treated animals. These results revealed that NO-mediated neurotoxicity due to intrahippocampal AlCl3 injection spread temporally and spatially to the forebrain cortex and suggested a potentially neuroprotective effect for L-NAME. Keywords aluminium chloride forebrain cortex L-NAME nitric oxide oxidative stress Introduction Neurodegenerative .
