Tuyển tập các báo cáo nghiên cứu khoa học trên tạp chí khoa học vật lý quốc tế đề tài: A Chemical Study on Phyllanthus reticulatus | Journal of Physical Science Vol. 19 2 45-50 2008 45 A Chemical Study on Phyllanthus reticulatus . Jamal . Yaacob and Laily B. Din School of Chemical Sciences and Food Technology Faculty of Science and Technology Universiti Kebangsaan Malaysia Bangi Selangor 43600 Malaysia Corresponding author jamal_nasser4@ Abstract A phytochemical study was conducted on the leaves of Phyllanthus reticulatus obtained from a riverside in Taman Negara Kuala Koh Kelantan. The separations of the chemical components were carried out using different chromatographic techniques and structures of compounds were elucidated by spectroscopic methods including nuclear magnetic resonance as well as mass spectrometry. Three compounds were isolated and identified as lupeol acetate stigmasterol and lupeol. Keywords Phyllanthus reticulatus leaves lupeol acetate stigmasterol lupeol NMR analysis 1. INTRODUCTION The Phyllanthus genus contains species which have useful medicinal applications. A considerable number of these species have been examined and some effective constituents have been reported. In particular the isolation of antineoplastic bisabolene glycosides phyllanthoside and phyllanthostatins from Phyllanthus accuminatus. Phyllanthus reticulatus is a large straggling or climbing shrub growing from 8 to 10 ft in The plant is used for a variety of ailments including smallpox syphilis asthma diarrhea and bleeding from Moreover it is also claimed the plant has antidiabetic activity in tribal area. In this paper the isolation and characterization of three known compounds from P. reticulates were reported. 2. EXPERIMENTAL RESULTS Thin layer chromatography TLC and preparative TLC were performed using pre-coated aluminium and glass plates with silica gel 60 F254 whereas column chromatography was carried out on silica gels 230-400 mesh. Spots and bands of compounds on TLC were detected using UV light. V spectra were recorded on a UV-1650PC spectrophotometer. Proton NMR .
