Tuyển tập các báo cáo nghiên cứu khoa học quốc tế về bệnh thú y đề tài: Translocational changes of localization of synapsin in axonal sprouts of regenerating rat sciatic nerves after ligation crush injury | J. Vet. Sci. 2000 1 1 1-9 OURNAL OF Veterinary Science Translocational changes of localization of synapsin in axonal sprouts of regenerating rat sciatic nerves after ligation crush injury Ku-birm Kwon Jin-suk Kim1 Byung-joon Chang Department of Anatomy and Histology Department of Pharmacology and Toxicology College of Veterinary Medicine Konkuk University Seoul 143-701 Korea Time-dependent translocational changes of Synapsin I SyI a synaptic vesicle-associated phosphoprotein and its involvement in the axonal transport were investigated in the regenerating axonal sprouts. A weak SyI immunoreactivity IR was found in the axoplasm of normal axons. Rat sciatic nerves were crush-injured by ligating with 1-0 silk thread at the mid-thigh level and released from the ligation 24 h later. At various times after release immunocytochemistry was performed. SyI was translocated from the proximal to the distal site of ligation and also involved in the sprouting of regenerating axons. The distribution patterns of SyI IR were changed in the crush-injured nerves. SyI immunoreactive thin processes were strongly appeared in the proximal region from 1 h after release. After 3 h a very strong IR was expressed. The intense SyI immunoreactive thin processes were elongated distally and were changed the distribution pattern by time-lapse. After 12 h strong immunoreactive processes were extended to the ligation crush site. At 1 day a very intense IR was expressed. At 2 days immunoreactive thin processes extended into the distal region over the ligation crush site and strong IR was observed after 3 days. SyI was accumulated in the proximal region at the early phases after release. These results suggest that SyI may be related to the translocation of vesicles to the elongated membranes by a fast axonal transport in the regenerating sprouts. Key words sciatic nerve regeneration immunocytochemistry Synapsin I. Introduction Illustration of the transported substances and the mechanisms involved in
