Tuyển tập các báo cáo nghiên cứu khoa học quốc tế về bệnh thú y đề tài: Detection of Lawsonia intracellularis in diagnostic specimens by one-step PCR | J. Vet. Sci. 2000 1 1 33-37 OURNAL OF Veterinary Science Detection of Lawsonia intracellularis in diagnostic specimens by one-step PCR Dong-Kyun Suh1 Suk-Kyung Lym2 You-Chan Bae2 Keun-Woo Lee3 Won-Pil Choi3 Jae-Chan Song3 Research Institute of Public Health and Environment Taegu 706-100 Korea National Veterinary Research and Quarantine Service Anyang 430-016 Korea College of Veterinary Medicine Kyungpook National University Taegu 702-701 Korea Lawsonia intracellularis is not culturable with a standard bacteriologic culture. One step PCR assay as a clinical diagnostic method was developed for the rapid detection of porcine proliferative enteritis PPE caused by L. intracellularis. Primers were designed based on the p78 DNA clone of L. intracellularis. The one step PCR resulted in the formation of a specific 210-bp DNA product derived from L. intracellularis. The nonspecific amplification product was not detected with swine genomic DNA or other bacterial strains causing similar symptoms to L. intracellularis infection. The one step PCR was as sensitive as 100 pg of L. intracellularis genomic DNA. We applied this method to field specimens diagnosed as PPE by macroscopic observation. Of 17 mucosal scraping specimens 16 94 were identified as positive to PPE and 15 88 of 17 feces specimens. These results suggest that the one step PCR can be used as a rapid diagnostic method for L. intracellularis infection. Key word Lawsonia intracellularis porcine proliferative enteritis diagnosis PCR. Introduction Porcine proliferative enteritis PPE known as ileitis intestinal adenomatosis or necrotic enteritis is a naturally occurring disease that can affect pigs from their weaning to young adult stage. PPE was formerly known to be caused by Camplyobacter-like organism or ileal symbiont intra-cellularis 5 15 . A recent work however have established that the causative agent was L. intracellularis an obligate intracellular bacterium 19 . The disease is of economic importance due to death
